Vps45 is a Sec1/Munc18-like (SM) protein that regulates SNARE complex formation in endosomal vesicle fusion. In yeast, Vps45 has been shown to interact with its binding partner SNARE Tlg2, the yeast homolog of mammalian syntaxin 16 (Sx16), to control its entry into the SNARE complex. Recent studies have identified two independent single amino acid substitutions in Vps45 associated with the immune disorder severe congenital neutropenia. These results suggest that wild-type and mutant Vps45 interact comparably with the cytoplasmic portion of Sx16/Tlg2, but one mutant may associate less strongly with a truncation. Future research should further characterize the mutants using these binding assays. An online introductory guide to biomedical grant proposal writing was also created.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• D'Ordine, Alexandra M

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042617-114306

Advisor

• Higgins, Lorraine D.
• Heilman, Destin

Year

• 2017

Sponsor

• University of Massachusetts Medical School

Date created

• 2017-04-26

Resource type

• Major Qualifying Project

Major

• Interdisciplinary

Rights statement

• In Copyright