In an attempt to advance pyrophosphate sequencing, a next generation sequencing technique, the multimeric state of Geobacillus stearothermophilus (Bst) Sulfurylase, an important enzyme in the technique, was investigated along with the extent in which it was biotinylated. The enzyme was determined to be a monomer with evidence of a small percent of enzyme possibly participating in higher order complexes. Between 45% and 57% of the enzyme was biotinylated.The investigation led to many discoveries and determined ways of improvement moving towards a more efficient technique, such as increasing the percent biotinylation and by changing the storage procedure to increase the enzyme shelf life.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Rock, Alexander Edward

Publisher

• Worcester Polytechnic Institute

Identifier

• E-project-042612-092608

Advisor

• Heilman, Destin

Year

• 2012

Sponsor

• Destin Heilman

Date created

• 2012-04-26

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright