Cyclin-dependent kinases (CDKs) are proteins that facilitate entrance of a cell through its cell cycle. Proteins p21 and p27 are CDK-inhibitors that arrest the cell cycle at the G0-G1 phase, and induce cell migration. The purpose of this MQP was to construct an expression system for biologically active p21 and p27. A TAT-mediated protein expression method was utilized for expression, and a HepG2 migration assay was developed to assay biological function. The data showed that recombinant proteins TAT-21 and TAT-27 were indeed biologically active in the migration assay.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Busby, Kristin Lori

Publisher

• Worcester Polytechnic Institute

Identifier

• 01D248M

Advisor

• Adams, David S.

Year

• 2001

Sponsor

• ViaCell

Date created

• 2001-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright