-Polyglutamic Acid has the potential to be used as a slow release drug delivery system. PGA conjugated to insulin was administered to insulin starved Reuber H35 rat hepatoma cells, and by GSK-3 phosphorylation, was shown to mimic physiological insulin. Through time course and dose response experiments we determined that 10M PGA-insulin and 25 minutes showed the maximum GSK-3 phosphorylation. Receptor ligand crosslinking was performed to determine if insulin bound to the receptor retained glutamic acid residues from the polymer.

• This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

Creator

• Ericson, Brett Richard
• Barford, Allyson Marie

Publisher

• Worcester Polytechnic Institute

Identifier

• 03D072M

Advisor

• Rulfs, Jill

Year

• 2003

Date created

• 2003-01-01

Resource type

• Major Qualifying Project

Major

• Biochemistry

Rights statement

• In Copyright