CHO Cell Dipeptide Transporter Metabolic Engineering Project
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open in viewerThis project investigated differences in the metabolism of CHO cells that had been modified to overexpress a dipeptide transporter (SLC15a1) and IgG1. SLC15a1 and control cultures were grown in media with either glutamine (Q) or alanine-glutamine (AQ) added over 10 days in shake-flasks. VCD and cell culture metabolic data were measured over the duration of the run. Results indicated that SLC15a1 cells grown in Q media maintained a higher VCD compared to the control, while results from the AQ SLC15a1 & control groups were indistinguishable. These observations suggest that SLC15a1 may be regulated by the concentration of monomer peptides such as Q present in media. The results may also suggest that SLC15a1 may be capable of weakly transporting glutamine as well as dipeptides.
- This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.
- Creator
- Publisher
- Identifier
- E-project-121118-005154
- Advisor
- Year
- 2018
- Date created
- 2018-12-11
- Resource type
- Major
- Rights statement
Las relaciones
- En Collection:
Elementos
Elementos
Miniatura | Título | Visibilidad | Embargo Release Date | Acciones |
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CHO_MQP_V.6.pdf | Público | Descargar | ||
CHO_MQP_V.6.docx | Público | Descargar |
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