Bile acid-induced apoptosis in a MDCK cell line transfected with the hepatocyte sinusoidal bile acid transporter

Perry, Leigh Ann

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Bile acid-induced apoptosis in a MDCK cell line transfected with the hepatocyte sinusoidal bile acid transporter

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The objective of this project is to determine if the canine kidney cell line (MDCK) transfected with the gene for the hepatocyte sinusoidal transporter (Ntcp) would be a useful model for rat hepatocytes in studying bile acid-induced apoptosis. In order to verify this cell line as a beneficial model, several experiments involving bile acids and cyclic adenine monophosphate were performed. A comparison of these results with those performed on hepatocytes indicate that MDCKs are valuable in studying bile acid-induced apoptosis.

This report represents the work of one or more WPI undergraduate students submitted to the faculty as evidence of completion of a degree requirement. WPI routinely publishes these reports on its website without editorial or peer review.

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